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Addgene inc trp1 selection marker
$A Growth of yeast cells (W303 strain, WT) carrying either the empty expression vectors (pYES2-URA3 and <t>pBF339-TRP1</t> vectors), or the galactose-inducible mouse BAX (pBM272-pGAL- BAX -URA3) and yeast Ate1 (pYES2-pGAL- ATE1-GFP -Ura3) in the presence of constitutively expressing Bcl-xL (pBF339-ADH- BCLxL -TRP1) or the vector (pBF339-TRP1) was measured by a serial dilution growth assay on either plate containing 2% glucose or 2% galactose, where the expression of Ate1 or BAX is not induced or induced, respectively. Plates were incubated at 30 °C and images were taken after 3 days. B Representative Western blots displaying changes in the distribution of cytochrome c (Cyc) between mitochondria and cytosol in yeast cell where Ate1-6 × His was induced for expression (“+Ate1”) with 2% galactose in Ura-minus liquid media for 6 h at 30 °C. The separated cytosolic fraction and mitochondrial fraction were compensated by buffer to be equal volume before analysis. Alpha-tubulin and Porin (VDAC) were used as markers to display the purity of cytosolic and mitochondrial fractions, respectively. Pgk1 was used as an additional loading control. The quantification of the blot was based on n = 6; error bars represent S.E.M. C Growth of yeast cells (WT or aif1 Δ) carrying either the empty expression pYES2 vector (“Vector”) or pYES-pGAL1: ATE1-GFP (“+Ate1-GFP”) was measured by a serial dilution growth assay on either plate containing 2% glucose or 2% galactose, where the expression of Ate1 is not induced or induced, respectively. Plates were incubated at 30 °C and images were taken after 3–5 days. D Similar to ( C ), except that WT, aif1 Δ, ate1 Δ¸ yca1 Δ, nuc1 Δ, and dnm11 Δ) yeasts were used. E Similar to ( C ), except that WT and cyc3 Δ yeasts were used. See also Suppl Fig. to see the equal growth rate of the involved mutant yeast strains carrying the empty vector on galactose-containing media compared to the WT strain, if they were not already shown in this figure.$
Trp1 Selection Marker, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trp1+markers/3413+pBF339+Bcl-XL+(Plasmid+%238773)/pmc12357888-394-39-45
Average 93 stars, based on 1 article reviews

trp1 selection marker - by Bioz Stars, 2026-10

93/100 stars

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1) Product Images from "Arginyltransferase1 drives a mitochondria-dependent program to induce cell death"

Article Title: Arginyltransferase1 drives a mitochondria-dependent program to induce cell death

Journal: Cell Death & Disease

doi: 10.1038/s41419-025-07917-1

$A Growth of yeast cells (W303 strain, WT) carrying either the empty expression vectors (pYES2-URA3 and pBF339-TRP1 vectors), or the galactose-inducible mouse BAX (pBM272-pGAL- BAX -URA3) and yeast Ate1 (pYES2-pGAL- ATE1-GFP -Ura3) in the presence of constitutively expressing Bcl-xL (pBF339-ADH- BCLxL -TRP1) or the vector (pBF339-TRP1) was measured by a serial dilution growth assay on either plate containing 2% glucose or 2% galactose, where the expression of Ate1 or BAX is not induced or induced, respectively. Plates were incubated at 30 °C and images were taken after 3 days. B Representative Western blots displaying changes in the distribution of cytochrome c (Cyc) between mitochondria and cytosol in yeast cell where Ate1-6 × His was induced for expression (“+Ate1”) with 2% galactose in Ura-minus liquid media for 6 h at 30 °C. The separated cytosolic fraction and mitochondrial fraction were compensated by buffer to be equal volume before analysis. Alpha-tubulin and Porin (VDAC) were used as markers to display the purity of cytosolic and mitochondrial fractions, respectively. Pgk1 was used as an additional loading control. The quantification of the blot was based on n = 6; error bars represent S.E.M. C Growth of yeast cells (WT or aif1 Δ) carrying either the empty expression pYES2 vector (“Vector”) or pYES-pGAL1: ATE1-GFP (“+Ate1-GFP”) was measured by a serial dilution growth assay on either plate containing 2% glucose or 2% galactose, where the expression of Ate1 is not induced or induced, respectively. Plates were incubated at 30 °C and images were taken after 3–5 days. D Similar to ( C ), except that WT, aif1 Δ, ate1 Δ¸ yca1 Δ, nuc1 Δ, and dnm11 Δ) yeasts were used. E Similar to ( C ), except that WT and cyc3 Δ yeasts were used. See also Suppl Fig. to see the equal growth rate of the involved mutant yeast strains carrying the empty vector on galactose-containing media compared to the WT strain, if they were not already shown in this figure.$
Figure Legend Snippet: A Growth of yeast cells (W303 strain, WT) carrying either the empty expression vectors (pYES2-URA3 and pBF339-TRP1 vectors), or the galactose-inducible mouse BAX (pBM272-pGAL- BAX -URA3) and yeast Ate1 (pYES2-pGAL- ATE1-GFP -Ura3) in the presence of constitutively expressing Bcl-xL (pBF339-ADH- BCLxL -TRP1) or the vector (pBF339-TRP1) was measured by a serial dilution growth assay on either plate containing 2% glucose or 2% galactose, where the expression of Ate1 or BAX is not induced or induced, respectively. Plates were incubated at 30 °C and images were taken after 3 days. B Representative Western blots displaying changes in the distribution of cytochrome c (Cyc) between mitochondria and cytosol in yeast cell where Ate1-6 × His was induced for expression (“+Ate1”) with 2% galactose in Ura-minus liquid media for 6 h at 30 °C. The separated cytosolic fraction and mitochondrial fraction were compensated by buffer to be equal volume before analysis. Alpha-tubulin and Porin (VDAC) were used as markers to display the purity of cytosolic and mitochondrial fractions, respectively. Pgk1 was used as an additional loading control. The quantification of the blot was based on n = 6; error bars represent S.E.M. C Growth of yeast cells (WT or aif1 Δ) carrying either the empty expression pYES2 vector (“Vector”) or pYES-pGAL1: ATE1-GFP (“+Ate1-GFP”) was measured by a serial dilution growth assay on either plate containing 2% glucose or 2% galactose, where the expression of Ate1 is not induced or induced, respectively. Plates were incubated at 30 °C and images were taken after 3–5 days. D Similar to ( C ), except that WT, aif1 Δ, ate1 Δ¸ yca1 Δ, nuc1 Δ, and dnm11 Δ) yeasts were used. E Similar to ( C ), except that WT and cyc3 Δ yeasts were used. See also Suppl Fig. to see the equal growth rate of the involved mutant yeast strains carrying the empty vector on galactose-containing media compared to the WT strain, if they were not already shown in this figure.

Techniques Used: Expressing, Plasmid Preparation, Serial Dilution, Growth Assay, Incubation, Western Blot, Control, Mutagenesis

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Article Title: ChEC-seq kinetics discriminates transcription factor binding sites by DNA sequence and shape in vivo
Article Snippet: The yeast ChEC-tagging vector pGZ108 was constructed by insertion of a PCR amplicon encoding a 3xFLAG tag and aa 83-231 of MNase (representing the mature sequence of MNase, GenBank P00644 ) followed by two stop codons (TAGTAG) between the PacI and AscI sites of pFA6a-3HA-kanMX6 (Addgene #39295), replacing the 3xHA tag.

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Article Title: ChEC-seq kinetics discriminates transcription factor binding sites by DNA sequence and shape in vivo
Article Snippet: .. ChEC-tagging vectors with the HIS3MX6 and TRP1 markers (pGZ109 and pGZ110), derived from pFA6a-3HA-HIS3MX6 (ref. ) (Addgene #41600) and pFA6a-3HA-TRP1 (ref. ; Addgene #41595), were also created but not used in this study. ..

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Strains and plasmids used in this study.

Journal: Microorganisms

Article Title: CesL Regulates Type III Secretion Substrate Specificity of the Enteropathogenic E. coli Injectisome

doi: 10.3390/microorganisms9051047

Figure Lengend Snippet: Strains and plasmids used in this study.

Article Snippet: pGBKT7 , Y2H vector containing GAL4 DNA binding domain; TRP1 nutritional marker , Clontech.

Techniques: Plasmid Preparation, Clone Assay, DNA Purification, Expressing, Mutagenesis, Derivative Assay, Amplification, Modification, Binding Assay, Marker, Activation Assay

Journal: Cell Death & Disease

Article Title: Arginyltransferase1 drives a mitochondria-dependent program to induce cell death

doi: 10.1038/s41419-025-07917-1

Figure Lengend Snippet: A Growth of yeast cells (W303 strain, WT) carrying either the empty expression vectors (pYES2-URA3 and pBF339-TRP1 vectors), or the galactose-inducible mouse BAX (pBM272-pGAL- BAX -URA3) and yeast Ate1 (pYES2-pGAL- ATE1-GFP -Ura3) in the presence of constitutively expressing Bcl-xL (pBF339-ADH- BCLxL -TRP1) or the vector (pBF339-TRP1) was measured by a serial dilution growth assay on either plate containing 2% glucose or 2% galactose, where the expression of Ate1 or BAX is not induced or induced, respectively. Plates were incubated at 30 °C and images were taken after 3 days. B Representative Western blots displaying changes in the distribution of cytochrome c (Cyc) between mitochondria and cytosol in yeast cell where Ate1-6 × His was induced for expression (“+Ate1”) with 2% galactose in Ura-minus liquid media for 6 h at 30 °C. The separated cytosolic fraction and mitochondrial fraction were compensated by buffer to be equal volume before analysis. Alpha-tubulin and Porin (VDAC) were used as markers to display the purity of cytosolic and mitochondrial fractions, respectively. Pgk1 was used as an additional loading control. The quantification of the blot was based on n = 6; error bars represent S.E.M. C Growth of yeast cells (WT or aif1 Δ) carrying either the empty expression pYES2 vector (“Vector”) or pYES-pGAL1: ATE1-GFP (“+Ate1-GFP”) was measured by a serial dilution growth assay on either plate containing 2% glucose or 2% galactose, where the expression of Ate1 is not induced or induced, respectively. Plates were incubated at 30 °C and images were taken after 3–5 days. D Similar to ( C ), except that WT, aif1 Δ, ate1 Δ¸ yca1 Δ, nuc1 Δ, and dnm11 Δ) yeasts were used. E Similar to ( C ), except that WT and cyc3 Δ yeasts were used. See also Suppl Fig. to see the equal growth rate of the involved mutant yeast strains carrying the empty vector on galactose-containing media compared to the WT strain, if they were not already shown in this figure.

Article Snippet: The inducible BAX-expression vector (pBM272-GAL-BAX-URA), with mouse BAX gene under Gal promoter and a URA3 selection marker, was acquired from Addgene (Plasmid #8770) The constitutively expression vector for Bcl-xL (pBF339-ADH-BCLxL-TRP), with mammalian Bcl-XL gene under ADH1 promoter and a TRP1 selection marker, was acquired from Addgene (Plasmid #8773).

Techniques: Expressing, Plasmid Preparation, Serial Dilution, Growth Assay, Incubation, Western Blot, Control, Mutagenesis

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